Identification of CD34+ and CD34- subpopulations of hematopoietic progenitor cells. BALB/c bone marrow cells were treated with Mouse BD Fc Block™ Purified Rat anti-CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) and stained with either FITC Rat IgG2a κ Isotype Control (Cat. No. 554688; data not shown) or FITC Rat anti-Mouse CD34 mAb RAM34 (Cat. No. 553733) and with either PerCP-Cy™ 5.5 Mouse Lineage Isotype Control Cocktail (Left Panel) or PerCP-Cy™ 5.5 Mouse Lineage Antibody Cocktail (Right Panel). Dead cells were excluded from analysis by staining with 7-AAD (7-Amino-Actinomycin D; Cat. No. 559925). Flow cytometry was performed on a BD LSR™II Flow Cytometry System.

Identification of CD34+ and CD34- subpopulations of hematopoietic progenitor cells. BALB/c bone marrow cells were treated with Mouse BD Fc Block™ Purified Rat anti-CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) and stained with either FITC Rat IgG2a κ Isotype Control (Cat. No. 554688; data not shown) or FITC Rat anti-Mouse CD34 mAb RAM34 (Cat. No. 553733) and with either PerCP-Cy™ 5.5 Mouse Lineage Isotype Control Cocktail (Left Panel) or PerCP-Cy™ 5.5 Mouse Lineage Antibody Cocktail (Right Panel). Dead cells were excluded from analysis by staining with 7-AAD (7-Amino-Actinomycin D; Cat. No. 559925). Flow cytometry was performed on a BD LSR™II Flow Cytometry System