淋巴细胞分离液是一种根据细胞密度差异,借助离心产生的重力加速度,进行细胞的分离纯化的常用试剂 。常用的淋巴细胞分离液有Ficoll淋巴细胞分离液
淋巴细胞分离液的原理:
外周血中单个核细胞和单核细胞,其体积、形态和密度与其他细胞不同.淋巴细胞分离液是一种密度介于1.075∽1.090之间而近似于等渗的溶液,用它做密度梯度离心,使一定密度的细胞按相应密度梯度分布,从而将各种血细胞加以分离。
基本应用 :
可用于体外分离外周淋巴血细胞,也可以从其他来源中分离单核细胞,包括脐带血细胞和骨、髓细胞,适用于从血液及组织 匀浆中分离所需细胞,在医疗和医学生物中广泛应用。其他人及动物多种比重细胞分离液。
Parameter | Ficoll-Paque PLUS |
Order Information | For research use only. |
Application | Cell isolation |
Density Max. | 1.078 g/ml |
Endotoxin Activity Max. | < 0.12 EU/ml |
Properties | Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood. |
Estimated shelf life from manufacture date | 3 years |
Storage conditions | 4–30°C |
Stability | Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4°C and 30°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate material in the solution |
Physical State | Liquid |
pH Range | 5.5–7.5 |
Color | Colorless to slightly yellow |
Certificate of Analysis | Yes |
Pack size | 6 x 100 mL/6X500 mL |
订购信息
货号 品牌
17144002 FICOLL PAQUE PLUS 6X100 ML
17144003 FICOLL PAQUE PLUS 6X500 ML